Fig. 1: Identification of Adipose-secreted signaling protein (Adissp).

a Strategies to identify brown adipocyte-secreted proteins. b Adissp-matched peptides identified by mass spectrometry are bold and underlined. c Adissp expression in wild-type (WT) mice tissues (n = 4). d Adissp expression in inguinal white adipose tissue (iWAT) from 2-month-old WT female mice administrated with PBS (n = 5) or CL-316,243 (CL) (n = 6). e Adissp expression in iWAT from WT mice housed at 22 °C (n = 5) or cold (4 °C) (n = 4) for 3 days from published data¹⁸. f Adissp mRNA expression in BAT and iWAT from 3-month-old male ob/ob mice (n = 3) and lean controls (n = 5). g Human ADISSP expression in different tissues (Bone marrow, Heart, Small Intestine, Kidney and Spleen, n = 4; Adipose tissue, Liver and Stomach, n = 3; Lung and Colon, n = 5) from published data¹⁹. h Expression of human ADISSP in subcutaneous adipocytes from lean (n = 10) and subjects with obesity (Men, n = 9; Women, n = 10) from published data²⁰. i Linear regression analysis of Body Mass Index (BMI) and ADISSP expression from adipose tissue of 770 men from published data²¹. j Detection of secreted Adissp-Flag from brown adipocytes containing a Flag knock-in. k Detection of Adissp secretion from brown adipocytes. N.S., non-specific band. l, Detection of Adissp secretion from brown adipocytes treated with Brefeldin A and Monensin for 6 h. m Subcellular localization of Adissp in brown adipocytes treated with or without CL-316,243 for 45 min. Scale bar, 20μm. n Detection of Adissp secretion from brown adipocytes treated with CL-316,243 at indicated time intervals. o Detection of Adissp secretion from brown adipocytes treated with oligomycin or Carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone (FCCP) for 1 h or 3 h. In Fig. 1j–o, experiments were repeated twice independently with similar results. Data are mean ± s.e.m. P values were determined by two-tailed Student’s t test (d, e, f and h) or Linear regression analysis (i). Source data are provided as a Source Data file.