Fig. 5: Nuclear USP18 regulates ISG landscape through IRF9/STAT2 and NFκB.
From: Expansion of interferon inducible gene pool via USP18 inhibition promotes cancer cell pyroptosis
a Scatter plots of H3K27ac tag counts at promoter/enhancer regions in WT vs. USP18−/−, WT vs. WT IFN, WT IFN vs. USP18−/− IFN, and USP18−/− vs. USP18−/− IFN THP-1 cells (n = 2 independent samples each). Red data points represent peaks with >2-fold change. b Heat map showing a comparative motif enrichment at enhancer regions defined by differential H3K27ac in (a). p-value was determined by two-tailed Hypergeometric test adjusted with Benjamini–Hochberg approach. c DNA pulldown assays with the indicated DNA probes and combinations of STAT2, IRF9, and USP18 proteins. d Relative firefly to renilla luciferase activity in U5A (IFNAR2−/−) cells expressing the indicated luciferase reporter constructs and combination of proteins. (n = 3 independent samples each). Data represent mean ± s.d. p-value was determined by one-way ANOVA test. e Genome browser tracks depicting ATAC and H3K27ac peaks in the atypical ISG PLK2. f Western blot analysis of the indicated proteins in cytoplasmic and nuclear fractions of USP18−/− THP-1 cells with or without IFNα (1000 U/ml) treatment. g IRF9 or p65 ChIP qPCR of the PLK2 and CCL4L2 promoters in WT or USP18−/− THP-1 cells with or without IFN treatment. (n = 3 independent samples each). Data represent mean ± s.d. p-value was determined by one-way ANOVA test. Source data are provided as a Source Data file.
