Fig. 1: Scheme of the MASP strategy which enables accurate, quantitative, and in-depth protein mapping across a whole tissue slice. | Nature Communications

Fig. 1: Scheme of the MASP strategy which enables accurate, quantitative, and in-depth protein mapping across a whole tissue slice.

From: In-depth mapping of protein localizations in whole tissue by micro-scaffold assisted spatial proteomics (MASP)

Fig. 1

a Robust tissue micro-compartmentalization with precisely-preserved spatial information, by a 3D-printed micro-scaffold. b Efficient/reproducible preparation of the location-specific micro-specimens (micro-surfactant-aided extraction/precipitation/on-pellet digestion, µ-SEPOD) followed by robust/sensitive LC-MS analysis. c Protein mapping by the MAsP app, which matches the accurately-measured protein abundance to the corresponding location of each micro-specimen. The app also generates customizable protein maps and offers various post-processing functions such as clustering or correlation of protein distribution patterns (more details are in the Methods and Supplementary Notes). The protein abundance scale is from low (green) to high (red).

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