Fig. 3: The high reproducibility and accuracy of the MASP pipeline. | Nature Communications

Fig. 3: The high reproducibility and accuracy of the MASP pipeline.

From: In-depth mapping of protein localizations in whole tissue by micro-scaffold assisted spatial proteomics (MASP)

Fig. 3

a Intra-group CV% of the quantitative values of 5019 quantified proteins among sample preparation replicates of a pooled quality control (QC) sample (analyzed once every 20 micro-specimens, N = 9). The box shows the 25th to 75th percentile range with the median indicated by a horizontal line. Whiskers extend to the 10th and 90th percentile range. b The correlation of quantitative values of 5019 quantified proteins between randomly-selected runs of the QC sample. c The agreement between the theoretical distribution and MASP-acquired map of strategically spiked non-endogenous peptides. d The maps of oligodendrocyte markers, Mbp, Mog, Osp, Cnp, and Plp, agreed well with the expected patterns and showed highly correlated distributions (Pearson correlation r = 0.78–0.99) (upper); the distribution maps of proteins highly-expressed in the cortex or hypothalamus (lower). e High correlation of the maps of the two distinct proteins that form the same heterodimeric protein complex, including the heterodimers of the tubulins and Na, K-ATPases. The z-score color scale for c–e is from −1.0 (green) to 1.0 (red). Source data are provided as a Source Data file.

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