Fig. 5: A3F and A3G reduce the number of integration hotspots and clustering of sites.
From: Antiretroviral APOBEC3 cytidine deaminases alter HIV-1 provirus integration site profiles
A Analysis of integration site hotspots. A hotspot was defined as a 1 kb window in the genome hosting 4 or more unique integration sites. Integration hotspots are shown as a proportion of total integration sites from CEM-SS cells infected with HIV-1 produced in the presence of no A3F or A3G (“No A3”) control (blue bars), or from cells expressing A3F-WT (black bars), A3F [E251A] (brown bars), A3G-WT (red bars), A3G [E259A] (green bars), A3G [W94A] (purple bars), or A3G [W127A] (yellow bars). B Integration site clustering was assessed by comparing the spacing between integration sites genome-wide to the same number of uniformly distributed (random) sites. Distances between sites are collected in seven length (L) ‘bins,’ with the shortest intersite lengths to the left and the longest to the right. A matched random control dataset was generated in silico (see methods for details). Fisher’s exact test, two-sided.
