Fig. 8: A3F and A3G promote latent infections in vitro.
From: Antiretroviral APOBEC3 cytidine deaminases alter HIV-1 provirus integration site profiles
A Schematic of the HIV-1 GKO reporter vector showing the csGFP gene (green box) under transcriptional control of the HIV-1 5’ LTR promoter and the mKO2 gene (orange box) under control of the constitutive EF1alpha promoter. B CEM-SS cells were infected with HIVGKO or HIVGKO-ΔU3LTR in the presence or absence of A3F, A3F[E251A], A3G, or A3G[E259A] for 48 h. Using flow cytometry, live cells were gated on using Zombie NIRTM staining and the percentage of double-positive (csGFP+, mKO2+) (blue bars) and single-positive cells ((csGFP−, mKO2+) (red bars) or (csGFP+, mKO2−) (gray bars)) is shown. Statistical analysis was performed using two-way ANOVA with Dunnett’s multiple comparisons test (degrees of freedom = 36). C The average proportion of latently infected cells (csGFP−, mKO2+) from panel B is shown. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparisons test (degrees of freedom = 12). Data shown represents the mean values (±S.E.M.) from three independent experiments. Source data are provided as a Source Data file.
