Fig. 4: Confinement of epithelial cell pairs on patterned holey carbon electron microscopy grids.
From: Morphological control enables nanometer-scale dissection of cell-cell signaling complexes
Micropatterns are patterned on the carbon film of EM grids and surrounded by a bio-passivated layer to prevent cell adhesion outside of the patterned area, coated with Oregon Green gelatin and then populated with PTK-1 cells expressing EGFP-alpha E catenin. a, c Phase contrast and b, d corresponding fluorescence images of patterned areas are shown in the top panels. e–g Representative cell doublet fully confined within the patterned area (e- phase contrast, f- fluorescence: DAPI in blue; Oregon Green gelatin and EGFP-alpha E catenin in green, and g- overlay of phase contrast and fluorescence images). Scale bars, 60 µm (a–d) and 20 µm (e–g). All ECM patterns are 66.5 × 66.5 µm.
