Fig. 3: Anti-SARS-CoV-2 IG repertoires.

A Frequencies of probe+ IgG or IgA B cells sorted for IG repertoire analysis. Symbol shapes indicate each donor as on legend for panel 1B. Results were obtained by a single measurement per sample. B Proportion of probe+ B cells binding to each domain. Bars represent means with standard deviations. Source data for A and B are provided as a Source Data file. C SARS-CoV-2-specific VH repertoire analysis by infecting variant WA1, Beta and Gamma shown in grey, orange and blue, respectively, with data from pre-pandemic controls in yellow. The x-axis shows all germline genes used; y-axis represents percent of individual gene usage. Horizontal lines show the median of each group for each gene. Red stars indicate genes with at least one significant difference between groups based on a Kruskal–Wallis test; pairwise comparisons using the Dunn test with correction for multiple testing are in Supplementary Table 3. n = 133, 737, 190, and ~7 × 10⁸ heavy chains from 4, 7, 2, and 3 individuals, for WA1-infected, Beta-infected, Gamma-infected, and historical controls, respectively. Combined frequency of VH genes capable of giving rise to stereotypical Y501-dependent antibodies (IGHV4-30, IGHV4-31, IGHV4-39, and IGHV4-61) in D Beta- or Gamma-binding B cells from individuals infected with each variant or E B cells from Beta-infected individuals sorted with either WA1- or Beta-derived probes. For both D and E, the boxes show the interquartile range, with the median marked as heavy horizontal band. Whiskers represent the highest (lowest) datapoint within 1.5 times the interquartile range of the 75th (25th) percentile. In panel D, n = 83, 349, and 60 cells from 4, 5, and 2 individuals for WA1-, Beta-, and Gamma-infection, respectively. In panel E, n = 111 and 349 cells from 5 individuals which bound to WA1 and Beta probes, respectively.