Fig. 6: The STN–LPB projection modulates pain-like behaviors.
From: A nigro–subthalamo–parabrachial pathway modulates pain-like behaviors
a, b Timeline and diagram for optogenetic manipulation of the STN–LPB projection. c Effect of activation of the STN–LPB projection on pain thresholds in ChR2-expressing mice. PWT: F(1, 24) = 12.02, P = 0.002; PWL: F(1, 12) = 10.46, P = 0.0035; n = 7 per group. d Effect of silencing of the STN–LPB projection on pain thresholds in NpHR-expressing mice. PWT: F(1, 22) = 0.4, P = 0.53; PWL: F(1, 22) = 0.14, P = 0.71; n ≥ 6 per group. e, f Effect of silencing of the STN–LPB projection on the frequency (F(1, 11) = 19.16, P = 0.0011) and duration (F(1, 11) = 44.53, P < 0.0001) of licking/flinching behavior induced by capsaicin hind paw injection. n = 7 in NpHR, n = 6 in eYFP. g–i Effect of silencing of the STN–LPB projection on PWT and PWL 15 min and 45 min after injection of capsaicin into hind legs. n ≥ 6 per group. g Baseline PWT, t = 0.99, P = 0.34; h F(1, 11) = 19.02, P = 0.0011; (i) F(1, 11) = 61.07, P < 0.0001. j, k Effect of silencing of the STN–LPB projection on PWT (F(2, 22) = 8.13, P = 0.0025) and PWL (F(2, 22) = 9.96, P = 0.001) 24 h after CFA injection in hind paws. n ≥ 6 per group. i–m Effect of silencing of the STN–LPB projection on PWT (F(2, 28) = 14.44, P < 0.0001) and PWL (F(2, 28) = 17.24, P < 0.0001) 2 weeks after SNI surgery. n = 8 per group. *P < 0.05. **P < 0.01; Two-way ANOVA with Tukey’s post-hoc analysis for (c–f) and (h–m); Two-tailed paired t test for (g). Data are presented as mean ± SEM.
