Fig. 6: In vitro studies of Cu@SA, Au@SA, and Au/Cu0@SA nanocubes. | Nature Communications

Fig. 6: In vitro studies of Cu@SA, Au@SA, and Au/Cu0@SA nanocubes.

From: Atomically dispersed golds on degradable zero-valent copper nanocubes augment oxygen driven Fenton-like reaction for effective orthotopic tumor therapy

Fig. 6

a The morphology of Au0.02Cu0.98@SA nanocubes treated with or without HepG2-Red-FLuc cancer cells as a function of time. b Cu+ release stained by CopperGreen dye showing green color as a function of time and the corresponding quantitative analysis. c H2O2 generation stained by hydrogen peroxide assay kit showing green color as a function of time and the corresponding quantitative analysis. d •OH generation stained by APF dye showing green color as a function of time and the corresponding quantitative analysis. e Analysis of hemolysis in blood containing 2% red blood cells from Au0.02Cu0.98@SA nanocubes. Negative and positive controls were conducted by immersing red blood cells in PBS and water, respectively. f Cytotoxicity analysis of vascular endothelial cells treated with Au0.02Cu0.98@SA nanocubes. The scale bar is 100 μm in all fluorescence images. All data were obtained in triplicate (n = 3, the error bars represented mean ± SD, and p-values were calculated by one-way ANOVA). Source data are provided as a Source Data file (one representative data was shown from three independently repeated experiments).

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