Fig. 1: St18 expression in the MGE and St18 GOF in in vitro model of MGE development.
From: St18 specifies globus pallidus projection neuron identity in MGE lineage
a E11.5 and b E13.5 parasaggital brain in situ for St18 (Allen Brain Atlas). MGE indicated by boxed areas. c E12.5 coronal brain RNAscope (green; DAPI blue) for St18, MGE indicated by boxed area, shown in higher power in (c’). d Schematic of transcriptional GOF in N/D and N/D/S conditions. N/D and N/D/S ES cell lines are differentiated over 12 days into EBs. Sectioned differentiated EBs from N/D and N/D/S shown below schematic with conditional fate-map (Dlx6aCre; Ai9) and immunolabeling for St18 and V5 tag. e 2D monolayer cultures from dissociated EBs shown with conditional fate-map (Dlx6aCre; Ai9, white). EBs are gently dissociated and then plated at high density to optimal survival and then cultured for 12 days before imaging. Immunohistochemistry and RNAscope repeated across 3 biological replicates. Scale bars: a–c 500 microns; d, e 100 microns.
