Fig. 3: St18 GOF produces projection-like neuronal morphologies.

a Experimental design of 2D monolayer tracing experiments. N/D and N/D/S lines are differentiated into EBs and dissociated into single-cell suspensions. Concordantly, P0 corticies are dissociated and combined with low-density EB suspensions for plating and whole-cell tracing following fate-mapping (Dlx6a^Cre; Ai9). b Sample N/D neuron plated at low density with fate-mapping (Dlx6a^Cre; Ai9). c Sample N/D/S neuron plated at low density with fate-mapping (Dlx6a^Cre; Ai9). Morphologcial differences noticeable throughout dataset, see Figure S2. d Representative tracings of N/D and N/D/S neurons. e Sholl Crossings, Process Length, Maximum Intersection Radius, and Enclosing Radius quantification (Unpaired t-test; Crossings p = 0.00000316; Enclosing Radius p = 0.0000638). f Sholl analysis curves for tracings in (d) and (e) (two-way ANOVA). Dataset represents 15 reconstructed neurons obtained from 4 separate differentiations per line. Data are presented as mean +/− SEM. Source data are provided as a Source data file.