Fig. 4: ASCs leave the ScAT and infiltrate injured muscle.

A Time course evaluation of murine in vitro ASC chemotaxis in response to plasma isolated from Ctrl, CTX- and Gly-injured animals at 1 dpi. n = 5 (Ctrl), 3 (Gly) and 4 (CTX) animals over three independent experiments. B In vitro human ASCs (of three individuals) chemotaxis in response to serum of six individuals collected 1 hour after an acute bout of continuous exercise (60% VO₂ max). C Correlation of human ASC chemotaxis with 1h-post exercise GDF-15 blood levels. n = 6 serums tested on 1 or 2 sets of ASC over three independent experiments. D Model of ScAT grafting from CD34-GFP mouse into WT C57Bl/6 mice (left panel), the figure was partly generated using Servier Medical Art, provided by Servier, licensed under a Creative Commons Attribution 3.0 unported license. Immunohistofluorescence image of the ScAT depot 7 days post-graft surgery (right panel, bar scale 200 μm). E Flow cytometry analysis of the SVF from Gly-injured muscle of the grafted mice (1 dpi), GFP⁺/CD45⁻/CD31⁻ are scated on an histogram for Sca-1 intensity. F–H Immunohistological analysis of Gly-injured (1 dpi) quadriceps in grafted mice with KikGR ScAT in situ (green arrowheads point KikGR⁺/CD140α⁺/CD45⁻ (F), KikGR⁺/ Podoplanin⁺/CD31⁻ cells (G) or KikGR⁺/CD140α⁺/Sca-1⁺ (H)). Bar scale 10 μm. Results are expressed as mean ± SEM; *p < 0.05.