Fig. 6: The interaction of blood platelets with ASCs is crucial for effective muscle regeneration. | Nature Communications

Fig. 6: The interaction of blood platelets with ASCs is crucial for effective muscle regeneration.

From: Adipose tissue is a source of regenerative cells that augment the repair of skeletal muscle after injury

Fig. 6

A Model of platelet depletion and ASCs injection. The figure was partly generated using Servier Medical Art, provided by Servier, licensed under a Creative Commons Attribution 3.0 unported license. B Platelet numeration time course in control and Gly-injured animals from 1 to 14 dpi. n = 15 (Ctrl) and three animals for each other time point over two independent experiments. C Time course of mRNA expression of myogenic genes in quadriceps muscle from control and Gly-injured animals with and without platelet depletion. n = 12–16 (Ctrl) and 5–8 at 3 dpi, 6–8 at 7 dpi, and 6–9 at 14 dpi animals over four independent experiments. D Immunohistological confocal images of quadriceps muscles at 7 dpi (Gly +/− platelet depletion). Bar scales 500 (left) and 50 (right) μm. E Immunohistological-based quantification of regenerative (WGA centronucleated fibers) muscle fibers at 7 dpi. n = 5 (Gly), 5 (Gly + αPLA) and 4 (Gly + αPLA + ASCs) animals over four independent experiments. F Size distribution of regenerating muscle fibers in Gly-injured (red), platelet-depleted (gray), and ASCs supplemented (green) animals at 14 dpi. n = 4 (Gly), 6 (Gly + αPLA), and 5 (Gly + αPLA + ASCs) animals over four independent experiments. G Time course of mRNA expression of intramuscular connective tissue (IMCT) genes in quadriceps muscle from control and Gly-injured animals with and without platelet depletion. n = 5 (Ctrl) and, 5–7 at 3 dpi, 3–4 at 7 dpi, 4 at 14 dpi animals over three independent experiments. H Immunohistological-based quantification of IMCT deposition in quadriceps muscle from control and CTX-injured animals with and without platelet depletion at 14 dpi. n = 27 (Ctrl), 10 (CTX), 28 (CTX + αPLA), and 30 (CTX + αPLA + ASCs) pictures of 4, 3, 4, and 4 animals in each group, respectively, over three independent experiments. Results are expressed as a percentage of control animals with mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001.

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