Fig. 1: Organoid PKD cysts expand under flow. | Nature Communications

Fig. 1: Organoid PKD cysts expand under flow.

From: Glucose absorption drives cystogenesis in a human organoid-on-chip model of polycystic kidney disease

Fig. 1

a Representative images of organoids on days following transfer to suspension culture (upper), with quantification (lower) of cyst incidence as a fraction of the total number of organoids (mean ± s.e.m. from n ≥ 4 independent experiments per condition; ****p < 0.0001). b Schematic of workflow for fluidic condition. c Time-lapse phase contrast images of PKD organoids under flow (0.2 dynes/cm2), representative of four independent experiments. d Average growth rates of control organoids (Ctrl org.), non-cystic compartments of PKD organoids (PKD org.), and cystic compartments of PKD organoids (PKD cysts) under flow (0.2 dynes/cm2). Each experiment was performed for 6 h. Cyst growth rate was calculated on an individual basis as the maximal size of the cyst during the time course, divided by the time point at which the cyst reached this size (mean ± s.e.m. from n ≥ four independent experiments; each dot represents the average growth rate of organoids in a single experiment. ****p < 0.0001).

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