Fig. 1: l-menthol upregulates PP6 in inflamed keratinocytes and relieves IMQ-induced skin inflammation. | Nature Communications

Fig. 1: l-menthol upregulates PP6 in inflamed keratinocytes and relieves IMQ-induced skin inflammation.

From: Targeting the transcription factor HES1 by L-menthol restores protein phosphatase 6 in keratinocytes in models of psoriasis

Fig. 1

a Structural formula of l-menthol. b Immunoblotting of PP6 in HaCaT cells treated with or without 10 μM l-menthol for 12 h in the presence or absence of 1 μg/ml R848. c Schematic of IMQ-induced psoriasis mouse model and 50 μg/day l-menthol treatment. d Representative photographs of the ears of ethanol (EtOH) or 50 μg/day l-menthol treated IMQ-induced mice (n = 6). e Ear thickness changes of IMQ-induced mice treated with EtOH or l-menthol (n = 6). f Representative hematoxylin and eosin (H&E) staining of the ears treated as in (d). Scale bar, 20 μm. g, h Acanthosis (g) and dermal cellular infiltrates (h) of ears treated as in (d) (n = 6). i Representative immunohistochemical staining of Ki67 in ears treated as in (d) (n = 6). Scale bar, 20 μm. j Quantitation of Ki67+ epidermal cells in ears treated as in (d). k Enzyme-linked immunosorbent assay (ELISA) for IL-23, TNF-α, and IL-6 in skin lesions derived from IMQ-induced mice with the treatment of EtOH or 50 μg/day l-menthol (n = 6). l Immunoblotting (left panel) and quantitation (right panel) of Pp6 expression in epidermis derived from EtOH or 50 μg/day l-menthol-treated mice after induction of skin phenotype by IMQ (n = 6). The data in (b, d–l) are representative of three independent experiments. Statistical analyses were performed by two-way ANOVA (e) and two-tailed Student’s t-test (b, d, f–l). All data were presented as mean values ± SEM. Specific p values are indicated in the figure. Source data are provided as a Source Data file.

Back to article page