Fig. 5: HES1 transcriptionally activated the expression of IGBP1.

a PP6-interacted proteins in HaCaT cells treated with 10 μM l-menthol in the presence of 1 μg/ml R848. b qPCR detection of indicated genes in HaCaT cells treated with or without 10 μM l-menthol for 12 h in the presence of 1 μg/ml R848. Results are presented as the ratio of indicated genes to the GAPDH relative to that in the control (n = 7). c Immunoblotting of indicated proteins in the epidermis from IMQ-induced Hes1^fl/fl mice and cKO mice treated with or without 50 μg/day l-menthol. d qPCR detection of Igbp1 in the epidermis from IMQ-induced Hes1^fl/fl mice or cKO mice (n = 5). The data were presented as the ratio of Igbp1 to GAPDH relative to that in Hes1^fl/fl mice. e WT and mutated promoter reporter constructs. HES1-binding site in IGBP1 promoter predicted by JASPR. f Luciferase activity in HaCaT cells transfected with or without HES1 siRNA (si-HES1) and the WT or point-mutated promoter reporter plasmid of IGBP1 (n = 4). g qPCR detection of indicated genes in HaCaT cells transfected with or without a HES1-overexpression plasmid (HES1). The data were presented as the ratio of indicated genes to GAPDH relative to that in control (n = 4). h Immunoblotting and quantitation of indicated proteins in HaCaT cells transfected with or without a HES1-overexpression plasmid (HES1) in the presence of 1 μg/ml R848. (n = 3). The data in (b–h) are representative of three independent experiments. Statistical analyses were performed by a two-tailed Student’s t-test. All data were presented as mean values ± SEM. Specific p values are indicated in the figure. Source data are provided as a Source Data file.