Fig. 6: IGBP1 promotes PP6 expression in keratinocytes. | Nature Communications

Fig. 6: IGBP1 promotes PP6 expression in keratinocytes.

From: Targeting the transcription factor HES1 by L-menthol restores protein phosphatase 6 in keratinocytes in models of psoriasis

Fig. 6

a Immunoblotting of HES1, IGBP1, and PP6 expression in HaCaT cells transfected with or without HES1 siRNA (si-HES1) and IGBP1 overexpression plasmid (IGBP1). b quantitation of PP6 expression in HaCaT cells transfected with or without HES1 siRNA (si-HES1) and IGBP1 overexpression plasmid (IGBP1) (n = 4). c Immunoblotting of IGBP1 and PP6 expression in HaCaT cells transfected with scramble siRNA (Ctr) or with IGBP1 siRNA (si-IGBP1). d Immunoblotting of PP6-immunoprecipitated proteins in HaCaT cells transfected with an HA-Ubiquitin plasmid and scramble siRNA (Ctr) or with IGBP1 siRNA (si-IGBP1). e Immunoblotting of IGBP1 and PP6 expression in HaCaT cells transfected with an empty vector (Vec) or an IGBP1 overexpression plasmid (IGBP1). f Immunoblotting of PP6-immunoprecipitated proteins in HaCaT cells transfected with an HA-Ubiquitin plasmid and an empty vector (Vec) or an IGBP1 overexpression plasmid (IGBP1). g Cartoon sketch of the molecular mechanism of l-menthol in psoriasis therapy. The data in (a–f) are representative of three independent experiments. Statistical analyses were performed by a two-tailed Student’s t-test. All data were presented as mean values ± SEM. Specific p values are indicated in the figure. Source data are provided as a Source Data file.

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