Fig. 2: Immunogenicity of one or two booster rS-Beta doses approximately 1 year after immunization with rS-WU1 in olive baboons. | Nature Communications

Fig. 2: Immunogenicity of one or two booster rS-Beta doses approximately 1 year after immunization with rS-WU1 in olive baboons.

From: Immunogenicity and protection of a variant nanoparticle vaccine that confers broad neutralization against SARS-CoV-2 variants

Fig. 2

A A small cohort of baboons (N = 2–3/group) were originally immunized with 1 µg, 5 µg, or 25 µg rS-WU1 with 50 µg Matrix-M adjuvant or unadjuvanted 25 µg rS-WU1 on day 0 and 21 (week 0 and 3, respectively). Approximately 1 year later, all animals were boosted with one or two doses of 3 µg rS-Beta with 50 µg Matrix-M adjuvant on day 318 and 339 (week 45 and 48, respectively). B Anti-S (WU1) IgG titers were measured throughout the course of the study. Individual animals’ titers are shown over time, different colored symbols and lines represent different dose groups for the initial rS-WU1 immunization series. Sera collected pre-rS-Beta boost (study week 43) as well as 7, 21, 35, 81, 137, 193, and 300 days after the first rS-Beta boost were analyzed to determine C anti-rS-WU1 (same data displayed in panel B), D anti-rS-Beta, and E anti-rS-Omicron BA.1 IgG titers by ELISA (n = 3). F Antibody titers capable of disrupting the interaction between rS-WU1, rS-Beta, or rS-Omicron and the hACE2 receptor by ELISA (n = 3) (gray bars represent means), and G antibody titers capable of neutralizing SARS-CoV-2 variants USA-WA1, Beta, Alpha, Delta, and Omicron with a PRNT assay (n = 3) (gray bars represent geometric means). H The presence of multifunctional CD4 + T cells positive for three Th1 cytokines (IFN-γ, IL-2, and TNF-α) was evaluated with intracellular cytokine staining after stimulation with rS-WU1 or rS-Beta (n = 2, gray bars represent means).

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