Table 1 Thermostability and hACE2 binding of SARS-CoV-2 recombinant spike proteins

From: Immunogenicity and protection of a variant nanoparticle vaccine that confers broad neutralization against SARS-CoV-2 variants

SARS-CoV-2 rS proteins

Differential scanning calorimetry (DSC)

hACE2 binding

Tmax (°C)

ΔHcal (kJ mol−1)

hACE2 binding kinetics by bio-layer interferometry

hACE2 ELISA (EC50, ng/mL)

Ka (1/Ms)

Kdis (1/s)

 

rS-Beta

67.88

1883.0

3.94 × 104

1.46 × 10−7

8.0

rS-WU1

63.21

1578.9

1.08 × 104

1.56 × 10−7

9.4

  1. Tmax melting temperature, Ka binding constant, Kdis dissociation constant, EC50 half-maximal binding.
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