Fig. 3: ΔyejABEF is hypersensitive to Ampicillin, in a manner only partly dependent on the β-lactamase AmpC. | Nature Communications

Fig. 3: ΔyejABEF is hypersensitive to Ampicillin, in a manner only partly dependent on the β-lactamase AmpC.

From: Peptidoglycan recycling mediated by an ABC transporter in the plant pathogen Agrobacterium tumefaciens

Fig. 3

A Genomic context of the AmpC β-lactamase in A. tumefaciens, showing proximity to canonical AmpR repressor. B Comparison of Amp resistance of A. tumefaciens WT, ΔyejABEF, ΔampC and ΔyejABEFΔampC strains by growth on LB plates with Ampicillin MIC test strips (μg/mL). C Hydrolysis of nitrocefin by lysates of A. tumefaciens WT, ΔyejABEF, ΔampC and ΔyejABEFΔampC strains, measured by following A486. n = 3 biologically independent experiments, error bars represent standard deviation from mean. Source data are provided as a Source Data file. D Comparison of Amp resistance of A. tumefaciens ΔyejA and ΔyepA (Atu1774) strains. E Accumulation of muropeptides in the supernatants of A. tumefaciens WT, ΔyepA, ΔyejA and ΔyejABEF strains, measured by UPLC. F Comparison of Aztreonam resistance of A. tumefaciens WT, ΔyejABEF and ΔampC strains by growth on LB plates with Aztreonam MIC test strips. G Minimal inhibitory concentration (MIC) values of A. tumefaciens WT, ΔyejABEF and ΔampC to β-lactams Ampicillin, Aztreonam, Mecillinam, Imipenem, and MurA-inhibitor Fosfomycin.

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