Fig. 4: PG recycling is required for cell wall integrity in A. tumefaciens.
A–E Phenotypic analysis of A. tumefaciens WT and ΔyejABEF strains. A Phase contrast microscopy of WT and ΔyejABEF cells grown on LB10 and LB0. Scale bar length is 5 μm. Images are representative of three biologically independent replicates. B Growth curves of WT and ΔyejABEF on LB10 and LB0. n = 3 biologically independent experiments, error bars represent standard deviation from mean. C Molar abundance of DD- and LD-crosslinks in cell walls determined by UPLC analysis of WT and ΔyejABEF PG. D Relative PG amounts determined by total area under UPLC chromatograms of WT and ΔyejABEF PG. E LC-MS quantification of primary soluble PG precursor UDP-M5 in WT and ΔyejB. F Volcano plot showing the ratio of Tn-Seq reads mapped to genes in the A. tumefaciens ΔyejABEF strain compared to WT as control. Selected synthetically detrimental (red) and synthetically beneficial (green) hits are highlighted. p−1 value determined from Mann–Whitney U test (threshold p−1 > 100). G Table listing selected synthetically detrimental or beneficial hits in ΔyejABEF Tn-Seq discussed in the main text, with annotations. Selected hits were genes putatively related PG homeostasis or cell division with significance value above threshold. Error bars in graphs represent standard deviation from mean. Source data for B–E are provided as a Source Data file.
