Fig. 1: Mapping of the projection of lateral PB neurons and characterizations of the lPB-PSTN pathway.

a Schematic of microinjection into the lateral PB (lPB) of the C57BL/6J mice. b Images of fluorescence at the injection site, lPB. Arrowheads point to the cell bodies of Chronos:GFP-expressing cells. scp, superior cerebellar peduncle. c, d Images of fluorescence at the projection sites of lPB neurons, PSTN (c) and CeA (d). cp, cerebral peduncle. Scale bars represent 200 μm. Experiments were repeated independently in at least two mice with similar results. e, f Schematic illustration and representative image of microinjection of CTB555, a retrograde tracer, into the PSTN of C57BL/6J mice. Scale bar represents 500 μm. g, h Representative image showing the retrogradely labeled PB neurons (g), and image merged with DAPI (h). Scale bars represent 50 μm (inset) or 500  μm. Experiments were repeated independently in at least three mice with similar results. i Bilateral injection of AAV-Syn-Chronos:GFP into lPB of C57BL/6J mice. j Traces of EPSCs (gray, 15 consecutive responses; red, average) evoked by photo-stimulation (every 20 s, 5-ms duration). Data are represented as mean + SEM. Scale bar, 50 pA and 10 ms. Right panel represents summary of the EPSC amplitudes (n = 8 cells). k Firing patterns of PSTN neurons (n = 21 cells). Scale bar, 20 mV and 200 ms. See also Supplementary Fig. 1.