Fig. 3: Characterization of AA-T3A-C12 LNP and cellular uptake.
From: Ligand-tethered lipid nanoparticles for targeted RNA delivery to treat liver fibrosis
a A representative TEM image of AA-T3A-C12/siRNA LNP from three independent experiments. Scale bar, 100 nm. b Flow cytometry analysis of cellular uptake of Cy5-siRNA-loaded LNPs with or without haloperidol (HP) pretreatment (representative dataset from n = 3/group). c Flow cytometry analysis of competitive cellular uptake of Cy5-siRNA-loaded LNPs in a fibroblast/hepatocyte (3T3-GFP/H2.35) co-culture environment (representative dataset from n = 3/group). The mean fluorescence intensity ratio between fibroblast and hepatocyte (MFI3T3-GFP/MFIH2.35) was calculated to indicate preferential uptake by fibroblasts over hepatocytes. Data are presented as mean ± SD. **p < 0.01; ***p < 0.001. b and c one-way ANOVA with Tukey’s correction. Source data are provided as a Source Data file. c was created with BioRender.com.
