Fig. 1: Chromatin structural variations during in vivo genome editing. | Nature Communications

Fig. 1: Chromatin structural variations during in vivo genome editing.

From: Safeguarding genome integrity during gene-editing therapy in a mouse model of age-related macular degeneration

Fig. 1

a Schematics for assessing chromosomal structural variations induced by dual-AAV-delivered SpCas9 in the mouse eye. b Editing efficiency for Vegfa-sg1 in mouse eye at 2-, 4- and 12-weeks post-transfection detected by PEM-seq. Error bars, mean ± SD (For 2-, 4-, and 12-weeks post-PBS injection mice, n = 8, 6, 6, respectively; for 2-, 4-, and 12-weeks post-AAV injection mice, n = 3, 3, 5, respectively). c Circos plot indicating translocations among on-target and off-targets of Vegfa-sg1 in mouse eye detected by PEM-seq. DNA sequences for on-target and off-targets of Vegfa-sg1 are shown in the table. Mismatches of DNA sequences between on-target and off-targets are in red. d Percentages for off-target translocations (left) and general translocations (right) in mouse eye cloned from Vegfa-sg1 at 2-, 4- and 12-weeks post-transfection detected by PEM-seq. Error bars, mean ± SD (For 2-, 4-, and 12-weeks post-AAV injection mice, n = 3, 3, 6, respectively). e Percentages of AAV integrations at Vegfa-sg2 normalized to editing events at 2-, 4- and 12-weeks post-transfection detected by PEM-seq. Error bars, mean ± SD (For 2-, 4-, and 12-weeks post-AAV injection mice, n = 3, 3, 6, respectively).

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