Fig. 3: Cas9TX nearly eliminates chromosomal translocations in vivo. | Nature Communications

Fig. 3: Cas9TX nearly eliminates chromosomal translocations in vivo.

From: Safeguarding genome integrity during gene-editing therapy in a mouse model of age-related macular degeneration

Fig. 3

a Circos plot indicating translocations among on-target and off-targets of Vegfa-sg1 in Cas9- and Cas9TX-treated mouse models of AMD detected by PEM-seq. b and c. Percentages for off-target translocations (b) and general translocations (c) cloned from Vegfa-sg1 in Cas9- and Cas9TX-treated mouse models of AMD detected by PEM-seq. Mean ± SD from three biological replicates. Two-tailed t-test, *p < 0.05, **p < 0.01. d Editing efficiency at Vegfa-sg2 in Cas9- and Cas9TX-treated mouse models of AMD detected by PEM-seq. Mean ± SD from three biological replicates. e and f Percentages for off-target translocations (e) and general translocations (f) cloned from Vegfa-sg2 in Cas9- and Cas9TX-treated mouse models of AMD detected by PEM-seq. Mean ± SD from three biological replicates. Two-tailed t-test, *p < 0.05, **p < 0.01.

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