Fig. 3: EIF4A3 locally inhibits METTL3 binding and m6A modification.
a Correlation between m6A fold change and METTL3 binding fold change upon EIF4A3 knockdown. Correlation coefficient (r) and P value was calculated by Pearson’s correlation analysis. b Average distribution of MeRIP-seq signal is shown, aligned around increased, decreased, and unchanged METTL3 binding peaks. c Boxplot showing the fold change of m6A enrichment on METTL3 binding peaks upon EIF4A3 depletion. METTL3 binding peaks are divided into three groups: no change, up regulated and down regulated upon EIF4A3 KD. Solid line represents median, with whiskers indicating minimum to maximum values. Statistical analyses, unpaired two-tailed Student’s t-test. d The length of internal exons with unchanged, increased or decreased METTL3 binding peaks. Solid line represents median, with whiskers indicating minimum to maximum values. Statistical analyses, unpaired two-tailed Student’s t-test. e The length of protein coding genes with unchanged, increased or decreased METTL3 binding peaks. The solid line represents median, with whiskers indicating minimum to maximum values. Statistical analyses, unpaired two-tailed Student’s t-test. f The exon number of genes with unchanged, increased, or decreased METTL3 binding peaks. Solid line represents median, with whiskers indicating minimum to maximum values. n = 1519; 224; 108. Statistical analyses, unpaired two-tailed Student’s t-test. g Genome Browser tracks of meRIP-seq and METTL3 eCLIP-seq read coverage at gene RFC5 and SNRPA in siCtrl and siEIF4A3 HeLa cells. h meRIP-qPCR and METTL3 CLIP-qPCR analysis showing increased m6A modification and METTL3 binding ability upon EIF4A3 KD. Data are mean ± S.E.M. of three or four independent experiments. Statistical analyses, two-tailed Student’s t-test. Source data are provided as a Source Data file.
