Fig. 3: Targeted methylation at MeSWEET10a shows high specificity.
From: Improving cassava bacterial blight resistance by editing the epigenome
A Distribution of CG, CHG, and CHH methylation over the targeted and flanking 5 kb regions of the MeSWEET10a promoter in wild-type TME419 and two independent DMS3-ZF lines (transgenic lines 133 and 204). Gray box indicates the targeted region within the MeSWEET10a promoter. ZF binding site is shaded within the pink box. Two detected hypermethylated differentially methylated regions (DMR) are also indicated. B Summary of CG, CHG, and CHH methylation level of targeted and flanking 5 kb regions of the MeSWEET10a promoter in wild-type TME419 and the two independent DMS3-ZF lines. C Whole genome CG, CHG, and CHH methylation level of wild-type TME419 and the two representative DMS3-ZF lines. D Metaplot of CG, CHG, and CHH methylation over the gene of protein coding genes (n = 33,653) and flanking 1 kb regions in wild-type TME419 and the two independent DMS3-ZF lines. Transcriptional start sites (TSS) and transcriptional termination sites (TTS) are indicated. Source data are provided as a Source Data file.
