Table 1 Summary of BarSeq experiments reported in this work

From: Quantifying the local adaptive landscape of a nascent bacterial community

Experiment

Libraries

Same flasks?

Description

怈fS怉

Mono

R, S, L

N

Library monoculture

Ā 

1:10 dil

R, S, L

N

Library monoculture, 1:10 daily dilution

Ā 

Glu exp

R, S, L

N

Library monoculture, kept in glucose exponential phase

Ā 

Ac exp

R, S, L

N

Library monoculture, kept in acetate exponential phase

Ā 

Eco Eq 1

S, L

Y

S + L libraries with wt L at ecological equilibrium

0.15

Eco Eq 2

S

N

S library with wt S + L at ecological equilibrium

0.17

Eco Eq 2

L

N

L library with wt S + L at ecological equilibrium

0.21

L in maj

S

N

S library with wt L in majority

0.08

S in maj 1

L

N

L library with wt S in majority

0.97

S in maj 2

S, L

Y

S + L libraries with wt S in majority

0.98

S in maj 3

L

N

L library with wt S in majority

0.97

  1. Dilution rate was variable in the glucose/acetate exponential phase experiments, to keep the populations in exponential phase (see methods section ā€œBarSeq experimentsā€), but unless otherwise noted, the daily dilution rate was 1:100, consistent with the LTEE condition. All experiments were performed in the LTEE media, DM25, except for the acetate exponential phase experiment. The abbreviations R, S, and L refer to REL606, and 6.5k S/L, respectively. In coculture experiments, 怈fS怉 is the total frequency of S, averaged overall timepoints and replicates.
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