Fig. 1: ScRNA-seq analysis of the developing hearts at embryonic and neonatal stages.

A Diagram of the MULTI-seq procedure. Each sample was stained with a unique MULTI-seq barcode before pooling and loading into the 10× Chromium for single cell capturing. The captured single cells were converted into libraries for sequencing and demultiplexing for downstream bioinformatic analyses. B Our scRNA-seq datasets include the named mouse strains, developmental stages, and heart zones. Figure 1A and part of Fig. 1B were created with BioRender.com. C, D Unsupervised clustering of CD1 and C57BL/6 scRNA-seq datasets. E The expression pattern of notable CD1 cardiac cell lineage genes. F UMAP plot of CD1 cells labeled by cell types.