Fig. 5: GABAB receptors are dynamically trapped at synaptic sites in hippocampal neurons.
From: Filamin A organizes γ‑aminobutyric acid type B receptors at the plasma membrane
a, b Representative TIRF images of SNAP-GABAB1 (magenta) co-transfected with Bassoon-GFP (a) or Homer-GFP (b) in hippocampal neurons and labeled with SNAP-549 (green). Images are representative of three independent experiments. Arrows indicate colocalization between GABAB1 and either Bassoon (a) or Homer (b) at synaptic sites. Insets, results of single-particle tracking applied to the regions delimited by the white boxes. Trajectories are shown as in Fig. 4b. c, d Frequency distributions of GABAB trajectories in hippocampal neurons classified in the four groups identified by the TAMSD analysis in basal and stimulated (GABA 100 µM; 5 min incubation) conditions. Data are obtained from primary hippocampal neurons expressing either Bassoon-GFP (c) or Homer-GFP (d) to separately analyze the behavior of pre- and postsynaptic GABAB receptors. Data are mean ± SEM. n = 6 and 4 cells (255 and 82 trajectories) for Bassoon in basal and stimulated condition, respectively, and 5 and 5 cells (74 and 236 trajectories) for Homer in basal and stimulated condition, respectively, examined over three independent experiments. *p < 0.05 vs. basal condition and #p < 0.05 vs. extra-synaptic compartment by two-tailed Mann-Whitney U test. Scale bars, 2 µm (images in a, b) and 500 nm (insets in a, b). Source data are provided as a Source Data file.
