Fig. 4: Heteroresistance capabilities of phoP-phoQ hybrid and mgrB complemented mutants.
From: Resolving colistin resistance and heteroresistance in Enterobacter species
The hybrid complement mutants were created by combining phoP and phoQ genes either of E. xiangfangensis RBK-17-0394-1 (Ex), E. bugandensis RBG-17-0246-1 (Eb), and E. roggenkampii RPB-17-0516-2 (Er). Plasmids harboring phoP-phoQ hybrids were transferred to Ex394ΔphoPQ. Complementation of the phoQ from the isolates exhibiting higher colistin heteroresistance i.e., Eb and Er to the ΔphoPQ mutant of E. xiangfangensis RBK-17-0394-1 resulted in the colistin resistance equivalent to respective parent strains. Complementation of the ΔmgrB mutant of E. xiangfangensis RBK-17-0394-1 with the mgrB from Eb (which has an identical amino acid sequence as of Er) resulted in slightly lesser inhibition due to changes in the binding energy (detailed in Supplementary Fig. 9). The MIC data is provided in the Source Data file.
