Fig. 3: Pore-forming activity of GSDMB.
From: Insights into the GSDMB-mediated cellular lysis and its targeting by IpaH7.8
a Electrostatic surface representation of NTD-GSDMB. The basic residues are colored in blue and shown in stick representations. b Cell death was assessed by LDH cytotoxicity assay in HEK293T cells. Mutations of the basic residues in positively charged regions decreased the NTD-mediated LDH release. All experiments were repeated at least three times (mean ± sd, n = 3 independent biological replicates). Bottom, the expression levels of GSDMB proteins. c Pore model of NTD-GSDMB. The residues in the oligomerization interface selected for mutation are shown in pink sphere representations. d Mutations of the residues in the oligomerization interface reduced the NTD-mediated LDH release. All experiments were repeated at least three times (mean ± sd, n = 3 independent biological replicates). Bottom, the expression levels of GSDMB proteins. Source data are provided as a Source Data file.
