Fig. 3: TAPE-seq analysis using PE2 and PE4 in different cell lines.
From: TAPE-seq is a cell-based method for predicting genome-wide off-target effects of prime editor
a–f Venn diagrams showing the number of and overlap between validated off-target sites in PE2-transfected cell lines and the off-target sites predicted by TAPE-seq using PE2 or PE4 with the following pegRNAs and cell lines: a HEK4 (+2 G to T) in HEK293T cells, b HEK4 (+3 TAA ins) in HEK293T cells, c HEK4 (+2 G to T) in HeLa cells, d HEK4 (+3 TAA ins) in HeLa cells, e HEK4 (+2 G to T) in K562 cells, and f HEK4 (+3 TAA ins) in K562 cells. g The number of off-target sites missed by the different versions of PE (PE2 or PE4) used for TAPE-seq. h The number of missed off-target sites in the different cell lines (HEK293T, HeLa, and K562) used for TAPE-seq. The bars represent the mean. Error bars indicate standard deviation (n = 6 independent experiments each represented by different color).
