Fig. 3: Benchmarking LoxCode barcoding in the yolk sac. | Nature Communications

Fig. 3: Benchmarking LoxCode barcoding in the yolk sac.

From: In vivo clonal tracking reveals evidence of haemangioblast and haematomesoblast contribution to yolk sac haematopoiesis

Fig. 3

a (i) Experimental design for barcoding experiments. Experimental mice received only one dose of 4-OHT (at either E6.5, E7.5, or E8.5). Created with BioRender.com. (ii) Barcoding window in reference to 4-OHT injection time. b Primitive erythrocytes/Haem relationships after Cdh5iCre induction at E7.5 ((i), 116 informative barcodes, n = 5 embryos) and Rosa26iCre induction at E7.5 ((ii), 212 informative barcodes, n = 3 embryos). c Biomass analysis using 5–9 elements complex barcodes of primitive erythrocytes/Haem relationships after Cdh5iCre ((i), 111 barcodes, n = 5 embryos) or Rosa26iCre ((ii), 211 barcodes, n = 3 embryos) induction at E7.5. d Biomass analysis of yolk sac (YS) and head macrophage (microglia (Mg)) relationships after Rosa26iCre induction at E7.5. 49 barcodes, n = 3 embryos.

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