Fig. 4: Investigating haematopoietic and endothelial lineage relationships. | Nature Communications

Fig. 4: Investigating haematopoietic and endothelial lineage relationships.

From: In vivo clonal tracking reveals evidence of haemangioblast and haematomesoblast contribution to yolk sac haematopoiesis

Fig. 4

a Induction of barcode formation at E6.5 using Rosa26iCre (15 barcodes, n = 4 embryos). (i) Heatmaps of all informative barcodes. (ii) Biological reproducibility of clonal outcomes. Colours represent the total number of independent embryos with the stated clonal outcome. Values in parentheses represent the percentage of independent embryos in which the clonal outcome was observed. (iii) Summary of contribution to the E10.5 yolk sac non-erythroid haematopoietic lineages biomass (based on 5–9 element barcodes [12 barcodes]). b Induction of barcode formation at E7.5 using Rosa26iCre (80 barcodes, n = 6 embryos). (i) Heatmaps of all informative barcodes. (ii) Biological reproducibility of clonal outcomes. Colours represent the total number of independent embryos with the stated clonal outcome. Values in parentheses represent the percentage of independent embryos in which the clonal outcome was observed. (iii) Summary of contribution to the E10.5 yolk sac non-erythroid haematopoietic lineages biomass (based on 5–9 element barcodes [77 barcodes]). c Induction of barcode formation at E8.5 induction using Cdh5iCre (136 barcodes, n = 12 embryos). (i) Heatmaps of all informative barcodes. (ii) Biological reproducibility of clonal outcomes. Colours represent the total number of independent embryos with the stated clonal outcome. Values in parentheses represent the percentage of independent embryos in which the clonal outcome was observed. (iii) Summary of contribution to the E10.5 yolk sac non-erythroid haematopoietic lineages biomass (based on 5–9 element barcodes [109 barcodes]). d Barcode distribution between the E10.5 yolk sac endothelial (Endo) and non-erythroid haematopoietic (Haem) lineages after Cdh5iCre induction at E7.5. 135 informative barcodes, n = 6 independent embryos. e Barcode distribution between the E10.5 yolk sac endothelial (Endo) and non-erythroid haematopoietic (Haem) lineages after Rosa26iCre induction at E7.5. 116 informative barcodes, n = 3 independent embryos.

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