Fig. 6: Identification of the in vivo haemangioblast and discovery of the haematomesoblast. | Nature Communications

Fig. 6: Identification of the in vivo haemangioblast and discovery of the haematomesoblast.

From: In vivo clonal tracking reveals evidence of haemangioblast and haematomesoblast contribution to yolk sac haematopoiesis

Fig. 6

a (i) Experimental design to assess lineage relationships between all mesoderm derivatives of the yolk sac. Created with BioRender.com. (ii) Barcoding window in reference to 4-OHT injection time. Experimental mice received only one dose of 4-OHT (at either E5.5, E6.5, or E7.5). b Induction of barcode formation at E7.5 induction using Cdh5iCre (469 barcodes, n = 3 embryos). (i) Heatmaps of all informative barcodes. (ii) Biological reproducibility of clonal outcomes. Colours represent the total number of independent embryos with the stated clonal outcome. Values in parentheses represent the percentage of independent embryos in which the clonal outcome was observed. (iii) Summary of contribution to the biomass of E10.5 yolk sac lineages (based on 5–9 element barcodes [464 barcodes]). c Induction of barcode formation at E6.5 using Rosa26iCre (300 barcodes, n = 9 embryos). (i) Heatmaps of all informative barcodes. (ii) Biological reproducibility of clonal outcomes. Colours represent the total number of independent embryos with the stated clonal outcome. Values in parentheses represent the percentage of independent embryos in which the clonal outcome was observed. (iii) Summary of contribution to the biomass of E10.5 yolk sac lineages (based on 5–9 element barcodes (based on 5–9 element barcodes [280 barcodes]). d Induction of barcode formation at E5.5 using Rosa26iCre (172 barcodes, n = 14 embryos). (i) Heatmaps of all informative barcodes. (ii) Biological reproducibility of clonal outcomes. Colours represent the total number of independent embryos with the stated clonal outcome. Values in parentheses represent the percentage of independent embryos in which the clonal outcome was observed. (iii) Summary of contribution to the biomass of E10.5 yolk sac lineages (based on 5–9 element barcodes (based on 5–9 element barcodes [163 barcodes]). Mes (mesenchyme), Endo (endothelium), EryP (primitive erythroid), Haem (HPC, megakaryocyte, and macrophage), HG (haemangioblast), HM (haematomesoblast), MA (mesenchymoangioblast), and MM (multi-outcome mesoderm).

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