Fig. 3: PRMT5 promotes a distinct gene expression signature in murine CLL-like cells resembling human RT.

a ScRNA-seq analysis of spleen cells in Eµ-PRMT5 (n = 5) and Eµ-TCL1 (n = 4) mice, visualized via UMAP and clustered according to K-means (n = 10). B cell (Cd19+, Ms4a1+, Cd79a+), T cell (Cd3+, Cd4+, Cd8+), Mono: monocyte (Cd14+, Itgam), and neutrophil (Cd177, Itgam) clusters were assigned as indicated, Supplemental Fig. 2E. Cell density is calculated as the 2d kernel density estimate mapped to color scale. b “CLL-like” cells with co-expression of Cd19 and Cd5 (UMI counts >0) identified in the spleen of both Eµ-PRMT5 and Eµ-TCL1 (mice from a), localized to specific cell clusters. c Relative gene expression of B-cell maturation and leukemogenic markers in the spleen cells of Eµ-PRMT5 and Eµ-TCL1 mice (from a). Gene expression is shown as the Log10-transformed expression where expression is size factor normalized UMI counts. d K-means clusters stratified by genotype. Clusters are distinguished by color and number. e Heatmap highlighting the top 50 cluster-specific genes among B-cell clusters enriched in Eµ-PRMT5 (3.4, 3.6) and Eµ-TCL1 (3.4) mice (from a), showing expression relative to additional B-cell clusters 3.2 and 3.8. Gene expression across 150 genes is shown as the average Log10-transformed expression in splenic B-cell clusters. Genes associated with leukemia and lymphoma are highlighted. Source data are provided as a Source Data file.