Fig. 2: Microbiome OTU and metabolite differences in FD.
From: Gut microbiome dysbiosis drives metabolic dysfunction in Familial dysautonomia
a OTUs responsible for observed partition around medoids (PAM) clusters/enterotypes identified by random forest analysis. OTUs defining cluster/enterotype 1 are shown above the dotted line. OTUs defining cluster/enterotype 2 are shown below the dotted line (centrality line = median, boxes = IQR, whiskers = 1.5 times IQR, dots beyond whiskers = outliers). b Metabolites showing significantly different abundances in FD patients compared to relatives (line = mean). c Targeted analyses of TMA in stool (line = mean). d Targeted analysis of TMAO in serum (line = mean). n = a, 48 patients, 51 relatives; b, stool, 54 patient, 58 relatives; serum, 49 patients, 53 relatives; c, d, 24 patient-relative pairs. Random forest analysis (a); Natural log (LN) transformed metabolite concentration, permutational paired t-testing (two-sided) with FDR correction (b, choline, mean t-statistic = 0.1485, df = 33, mean difference between patient-relative pairs = 0.9860, mean 95% confidence interval of difference between patient-relative pairs = 0.5281 to 1.4440; xanthine, mean t-statistic = 2.9527, df = 33, mean difference between patient-relative pairs = −0.9591, mean 95% confidence interval of difference between patient-relative pairs = −1.4051 to −0.5130; urea, mean t-statistic = 0.9269, df = 33, mean difference between patient-relative pairs = 0.7281, mean 95% confidence interval of difference between patient-relative pairs = 0.3527 to 1.1036; methanol, mean t-statistic = −1.5497, df = 33, mean difference between patient-relative pairs = −0.7295, mean 95% confidence interval of difference between patient-relative pairs = −1.1875 to −0.2716); Paired t-testing, two-sided) (c, TMA, t-statistic = 1.5423, df = 16, difference between patient-relative pairs = 0.4499, 95% confidence interval of difference between patient-relative pairs = −0.1685 to 1.0682; d, TMAO, t-statistic = 3.3197, df = 23, difference between patient-relative pairs = 0.6418, 95% confidence interval of difference between patient-relative pairs = 0.2419 to 1.0417).
