Fig. 6: Maraviroc prevents bone loss caused by aged TCNs. | Nature Communications

Fig. 6: Maraviroc prevents bone loss caused by aged TCNs.

From: TGFβ1+CCR5+ neutrophil subset increases in bone marrow and causes age-related osteoporosis in male mice

Fig. 6

a μCT 3D reconstruction of trabecular bone and coronal sections of tibial metaphyses of NSG mice injected with TCNs from aged C57 mice plus vehicle or maraviroc treatment. Bar = 1 mm. b–d Analysis of microstructure parameters in trabecular bones in (a), including trabecular bone volume (BV/TV) (b), number (Tb.N) (c) and separation (Tb.Sp) (d), and of bone mineral density (BMD) (e). Mean ± SD (n = 3, 4, and 4 biologically independent male mice for control, A-TCN and A-TCN plus maraviroc groups, respectively). f Representative images of TRAP- and H&E-stained tibial paraffin sections from NSG recipients as in (a) Bar = 50 μm. g–i Osteoblast surfaces (Ob.S/BS) (g), osteoclast surfaces (Oc.S/BS) (h), and numbers (Oc.N/BS) (i) on metaphyseal trabecular surfaces. Mean ± SD (n = 3, 4, and 4 biologically independent male mice, respectively). Analyses: one-way ANOVA with Tukey’s post hoc test. Source data are provided as a Source data file.

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