Fig. 10: EXO1 deficiency leads to heightened chromosomal breaks, quadriradial chromosomes, and accumulation of 4 N DNA. | Nature Communications

Fig. 10: EXO1 deficiency leads to heightened chromosomal breaks, quadriradial chromosomes, and accumulation of 4 N DNA.

From: A CRISPR-Cas9 screen identifies EXO1 as a formaldehyde resistance gene

Fig. 10

a Survival curve of mouse embryonic fibroblasts (MEFs) Exo1 WT and Exo1 KO treated with different concentrations of formaldehyde for 96 h. Data are presented with ±SEM from three independent experiments. b Survival curve of MEFs Exo1 WT and EXO1 KO treated with different concentrations of MMC for 96 h. Data are presented with ±SEM from three independent experiments. c Quantification of chromosomal breaks and quadriradial chromosomes per metaphase in MEFs Exo1 WT and Exo1 KO MEFs under 0 or 90 nM MMC for 48 h. **p < 0.01 and ****p < 0.0001 (one-way ANOVA, followed by Kruskal–Wallis test). d Percentage of cells with DNA content above 4 N in RPE-1 WT and EXO1 KO 11 cells after 24 h of 75 nM MMC treatment. e A potential model of how EXO1 participates in response to formaldehyde-induced DNA damage. Formaldehyde induces ICLs/DPCs, which are acted upon by the EXO1 and the FA pathway. When EXO1 or FA pathways are inactivated, this leads to aggravated DNA replication stress and DSB formation. The EXO1 or FA pathways cannot compensate totally for each other as they show additive functions in epistatic analyses.

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