Fig. 3: ATF3 deficiency prevents steatosis-induced necroptosis after hepatectomy. | Nature Communications

Fig. 3: ATF3 deficiency prevents steatosis-induced necroptosis after hepatectomy.

From: The transcription factor ATF3 switches cell death from apoptosis to necroptosis in hepatic steatosis in male mice

Fig. 3

a, b Mice fed normal chow (NC) or a HFD for 2 or 16 weeks underwent partial hepatectomy. a Immunoblot analysis. b Quantitative PCR analysis. c–j A-KO mice and littermates (CTRL) fed a HFD for 16 weeks underwent partial hepatectomy. c Haematoxylin-eosin staining. Scale bar, 50 μm. Asterisks indicate hepatocellular death foci. d Plasma ALT levels. e TUNEL staining. Scale bar, 50 μm. Arrowheads indicate solitary dead hepatocytes. Asterisks indicate hepatocellular death foci. Arrows indicate perifocal dead hepatocytes. f Number of TUNEL+ hepatocytes defined as described. g TUNEL/Cl-CASP3 double staining (left). Cl-CASP3+ or Cl-CASP3− hepatocytes as a percentage of TUNEL+ hepatocytes (right). h Quantitative PCR analysis. i Immunoblot analysis. j Hepatic MDA levels. Data are presented as the mean values ± SEM. [(a, b) n = 5/group; (c–j) n = 6/group, biologically independent samples]. Statistics: one-way ANOVA followed by Tukey’s multiple comparisons test (b), one-way repeated-measures ANOVA followed by Bonferroni’s multiple comparisons test (d), two-tailed Student’s t test (f–h, j). D, diet effect; T, time effect; G, group effect. Source data are provided as a Source Data file.

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