Fig. 8: RIPK3 knockdown ameliorates NASH induced by MCD feeding.

a, b Wild-type mice were treated as indicated. a Immunoblot analysis. b Quantitative PCR analysis. c, d ISH for Atf3 and Ripk3. c ISH images of serial sections. Scale bar, 50 μm. Arrows indicate both Atf3⁺ and Ripk3⁺ hepatocytes. Arrowheads indicate Atf3⁺ or Ripk3⁺ hepatocytes. Asterisks indicate hemosiderin pigmentation. d Percentage of Ripk3⁺ or Ripk3⁻ hepatocytes in Atf3⁺ hepatocytes (left). Percentage of Atf3⁺ or Atf3⁻ hepatocytes in Ripk3⁺ hepatocytes (right). e–j Ripk3 siRNA was injected into the mice at 2 and 4 weeks after MCD feeding. e Plasma ALT levels. f TUNEL staining (left). Scale bar, 50 μm. Number of TUNEL⁺ hepatocytes (right). g Sirius red staining. Scale bar, 50 μm. h Quantitative PCR analysis of genes related to fibrosis. i Immunoblot analysis. j Quantitative PCR analysis of Ripk3, Atf3 and Tnf. Data are presented as the mean values ± SEM. [(a–d) n = 6/group; (e–j) n = 5/group, biologically independent samples]. Statistics: one-way ANOVA followed by Tukey’s multiple comparisons test (b), two-tailed Student’s t test (d, f, h, j), one-way repeated-measures ANOVA followed by Bonferroni’s multiple comparisons test (e). PHx, post-hepatectomy; R-KD, Ripk3 knockdown; D, diet effect; G, group effect; T, time effect. Source data are provided as a Source Data file.