Fig. 2: Presynaptic IL-13 and a postsynaptic lL-13Ra1 demonstrated by super-resolution microscopy.
From: Interleukin-13 and its receptor are synaptic proteins involved in plasticity and neuroprotection
a Distinct separation between PSD-95 and IL-13 visible in STED images of single synapses. b STED imaging and intensity profile plots of single synapses show an overlap between PSD-95 and IL-13Ra1. c STED imaging and intensity profile plots of single synapses show an overlap between Bassoon and IL-13. d Distinct separation between Bassoon and IL-13Ra1 visible in STED images of single synapses. e Distribution of distance between IL-13 and IL-13Ra1 peaks compared to PSD-95 reveals that IL-13 peak is distal to PSD-95, the IL-13Ra1 overlaps with the PSD-95 peak. f Distribution of distance between peaks reveal an overlap between IL-13 and Bassoon, whereas IL-13Ra1 peak is distinct at −150 nm from Bassoon. g STED imaging and intensity profile of single synapses show a separation between IL-13Ra1 and IL-13. h Distribution of distance of IL-13 and IL-13Ra1 reveals that the two peaks do not overlap and are located at ~150 nm. i Distribution of the separation of IL-13Ra1 and IL-13 in reference to the MAP2 + dendritic profile reveals that IL-13Ra1 peak is proximal, whereas IL-13 peak is distally located, in agreement with post- and presynaptic localization. j IL-13 shows 72% synaptic colocalization with IL-13Ra1. a–j In all experiments N = 3; n = 200 synapses (19 dendrites for j). Scale bar overview: 1 μm, scale bar insert: 500 nm. AU: arbitrary units. Source data are provided as a Source Data file.
