Fig. 8: IL-13 reduces excitotoxic neuronal death.
From: Interleukin-13 and its receptor are synaptic proteins involved in plasticity and neuroprotection
a–c Significant reduction of glutamate (20 μM) induced neuronal toxicity after IL-13 treatment (50 ng/ml) in rat cortical neurons revealed in holotomography microscopy live imaging (p = 0.0003). Treatment with JAK (Ruxolitinib; 280 nM) and STAT6 (AS1517499; 1 μM) inhibitors show that the protective effects of IL-13 are dependent on a JAK/STAT6 dependent mechanism (IL-13 vs Rux: p = 0.0030; IL-13 vs AS: p = 0.0006). N = 4; n = C: 40; 20 μM: 35; 20 μM + IL-13: 37; AS: 36; Rux: 36 neurons. d, e High dose IL-13 (450 ng/ml) does not prevent to glutamate induced neuronal toxicity (IL-13 450 ng/ml vs IL-13 50 ng/ml: p = 0.0002). N = 4; n = C: 41; 20 μM: 41; 20 μM + IL-13 50 ng/ml: 33; 20 μM + IL-13 450 ng/ml: 36 neurons. 0.1% BSA + 0.1% DMSO used as vehicle control. Scale bar: 20 μm. **: p < 0.01; ***: p < 0.001. b, c, e Log-rank (Mantel–Cox) test. Source data are provided as a Source Data file.
