Fig. 6: Brain protection of C-dot SOD nanozymes against middle cerebral artery occlusion (MCAO)-induced ischemic and reperfusion injury.
From: Deciphering the catalytic mechanism of superoxide dismutase activity of carbon dot nanozyme
a Half-life analysis of C-dot SOD nanozymes in plasma (n = 3 mice). b Ex vivo fluorescence imaging analyses of the accumulations of C-dot SOD nanozymes (labeled with Cy5.5) in the brains of sham (24 h post-injection) and MCAO mice (2 h, 6 h, and 24 h post-injection), and corresponding brain sections (n = 3 mice). c The cerebral infarcted area analyses of MCAO mice treated with different dosages of C-dot SOD nanozymes for 24 h (n = 3 mice). d Representative 2,3,5- triphenyltetrazolium chloride-stained brain sections and (e) quantification of cerebral infarct areas of MCAO mice treated with different C-dot nanozymes (n = 3 mice). f Neurological score analyses of the MCAO mice treated with different C-dot nanozymes for 24 h (n = 5 mice). g Representative images of TUNEL staining in the brain sections (n = 3 mice, scale bar = 25 μm), h Malondialdehyde (MDA) assay in the brain homogenate, and ELISA assay of inflammatory factors (i) TNF-α, (j) IL-1β and (k) IL-6 of the infarcted brain of MCAO mice treated by different C-dot nanozymes (n = 3 mice). P values are determined with one-way ANOVA Tukey’s multiple comparisons test. Source data are provided as a Source Data file.
