Table 1 Details of antibodies used for multiplexed Immunohistochemistry

From: Single-cell analysis reveals prognostic fibroblast subpopulations linked to molecular and immunological subtypes of lung cancer

Target

Clone

Supplier

Product code

Dilution

Ag retrieval pH

CD31

JC70A

Agilent/Dako

IR61061-2

5x (RTU)

High

Pan-CK

AE1/AE3

Agilent/Dako

IR05361-2

5x (RTU)

High

MCAM

Polyclonal

SIGMA/Merck

HPA008848

500x

Low

ACTA2 (αSMA)

1A4

Agilent/Dako

IR61161-2

1x (RTU)

High

POSTN

Polyclonal

SIGMA/Merck

HPA012306

50x

Low

CD34

QBEnd 10

Agilent/Dako

M716501-2

50x

Low

AOC3

#393112

R&D Systems

MAB3957

500x

Low

HSPA1A

3A3

Santa Cruz

sc-32239

250x

Low

  1. RTU product purchased at supplier designated “ready to use“ concentration.
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