Fig. 3: Editing efficiency of the PE3 and PE5 systems upon recruitment of P65 through the MS2 system. | Nature Communications

Fig. 3: Editing efficiency of the PE3 and PE5 systems upon recruitment of P65 through the MS2 system.

From: Enhancement of a prime editing system via optimal recruitment of the pioneer transcription factor P65

Fig. 3

a Diagrams of the MS2-P65 fusion protein constructs and an overview of the MS2-P65-recruiting PE3 or PE5 (PE3 + MLH1dn) system. b Prime editing of genomic DNA in HEK293T cells by the MS2-P65-recruiting PE3 and PE5 systems. The editing efficiencies reflect the sequencing reads that contained the intended edits and did not contain indels among all treated cells, with no sorting. The gray bar indicates the indel frequency coupled with the editing efficiency indicated by the left closest bar. Bars represent the mean of n = 3 independent replicates. The error bars in all figures represent the SDs. The P values were calculated by a two-tailed t test. **P < 0.01; *P < 0.05. c Summary of PE3 and PE5 editing enhancement with the recruitment of p65. Bars represent the mean of n = 3 independent replicates. The error bars in all figures represent the SDs. Source data are provided as a Source Data file.

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