Fig. 2: Hepatocytes in SRSF1 HKO mice eventually circumvent knockout via AlbCre repression.
From: Splicing factor SRSF1 deficiency in the liver triggers NASH-like pathology and cell death
a Left: Representative IF images of liver sections from Control and SRSF1 HKO mice at indicated timepoints (n = 3 biologically independent animals, 6 fields per replicate). IF images show co-staining with SRSF1 (green), HNF4α (red), and Nuclei (blue). Right: Quantification of the fraction of SRSF1-expressing hepatocytes per field from IF images at each timepoint. b Relative mRNA expression (qPCR) of AlbCre normalized to 36B4 expression in Control and SRSF1 HKO mice (n = 4 mice per group) at the indicated timepoints. PN4, post-natal day 4. Two-way ANOVA analysis with multiple testing correction was performed to determine the significance between 2 groups at 2 timepoints. Significant differences are designated by “***” with the p-value listed above. Source data are provided as a Source Data file.
