Fig. 4: foxG is expressed in regenerating blastemas and regulates wnt1 expression.

a Schematic illustration indicating the foxG (RNAi) procedure. b WISH of wnt1 in foxG (RNAi) animals demonstrate its absence in both 12 and 72 hR wounds. Schematic illustration of wnt1 in intact animals with the analyzed zones (squares) added. Data representative of two independent experiments. c WISH of foxG in intact animals shows its expression in the posterior midline, similar to wnt1 expression. Data representative of one experiment. Orange dashed lines show the magnified area. Single cell analysis performed by⁶³ showed six genes (top 16%) over represented in posterior organizing wnt1+ cells. Among these, foxG was found. d WISH of wnt1 and foxG in regenerating anterior and posterior wounds at 24 hR showing a salt-and-pepper pattern (left panel). A magnification was taken from each blastema (right panel). Purple boxes show the magnified area. Schematic illustration of regenerating animals was added. Data representative of one experiment. Scale bar: 50 µm and 10 µm for left and right panel in b, respectively; 100 µm in c; 200 µm in d.